Comparison of Various Concentrations of Carbol Fuchsin Solutions in Ziehl-Neelsen Staining for Detection of Acid-Fast Bacilli in Sputum Samples

Comparison of Various Concentrations of Carbol Fuchsin Solutions in Ziehl-Neelsen Staining for Detection of Acid-Fast Bacilli in Sputum Samples

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This study was carried out to evaluate Ziehl-Neelsen staining using various concentrations of carbol fuchsin solution.Duplicate smears from 173 sputum samples were made and stained with 1%, 0.3% and 0.

1% carbol fuchsin solution.The smear results were recorded semi quantitatively according hp 72p to the WHO guidelines.A comparative analysis of the results obtained from each carbol fuchsin solution was carried out the McNemar test and p-value was determined.

P-value of <0.05 was considered significant.Comparison of 1% and 0.

3% carbol fuchsin solution shows close relationship with McNemar chi square of 3.0 and p-value of 0.39.

The agreement from these results had a kappa value of 0.934.Twenty-five (25) of the 173 samples were positive and detected by 1% and 0.

3% concentrations, 25(14%) and 25(14%) respectively.Comparison of 1% and 0.1% carbol fuchsin solution indicates a very poor relationship with a McNemar of 18 and a significant p-value of 0.

012.Twenty-five (25) of the 173 samples were positive which was detected by 1% 25(14%) and 0.1% detected only 20(11%).

The sensitivity of ZN using 0.3% and 1% basic fuchsin was comparable, while it was reduced using 0.1% compared to 1% basic fuchsin.

Reducing the concentration of basic fuchsin below 0.3% in ZN staining was found to significantly reduce spyderco urban its sensitivity.